Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR.

Solution

Role of Heat : - It helps in denaturation process in PCR. The ds DNA is heated in this process at very high temperature (95ºC) so that both the strands separates. Role of primers : - Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of new chain. Role of Bacterium Thermus aquaticus : - From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms new strand.

Some More Questions From Molecular Basis of Inheritance Chapter

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Molecular Basis Of Inheritance

Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR.

Some More Questions From Molecular Basis of Inheritance Chapter

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