(a) Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
(b) Write the use of restriction endonuclease in the above process.

Solution

(a) The palindromic sequences i.e. the sequence of base pairs read the same on both the DNA strands, when orientation of reading is kept the same.
(b) On finding the palindrome, the endonuclease binds to the DNA.
* It cuts the opposite strands of DNA, but between the same bases on both the strands & forms STICKY ENDS. This sticky ends facilitate the action of enzyme DNA ligase and helps in the formation of recombination DNA.

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Molecular Basis Of Inheritance

(a) Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
(b) Write the use of restriction endonuclease in the above process.

Some More Questions From Molecular Basis of Inheritance Chapter

Why was RNA unstable ?

What made DNA more preferrable as genetic material ?

Process of replication is completed in how much time in E.coli ?

What is average rate of polymerization ?

List three components of transcription unit.

Which RNA acts as an adapter ?

Name three kinds of polymerases.

What is splicing ?

Which is more stable DNA or RNA?

Expand UTR.

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Molecular Basis Of Inheritance

(a) Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA. (b) Write the use of restriction endonuclease in the above process.

Some More Questions From Molecular Basis of Inheritance Chapter

Why was RNA unstable ?

What made DNA more preferrable as genetic material ?

Process of replication is completed in how much time in E.coli ?

What is average rate of polymerization ?

List three components of transcription unit.

Which RNA acts as an adapter ?

Name three kinds of polymerases.

What is splicing ?

Which is more stable DNA or RNA?

Expand UTR.

Mock Test Series

NCERT Sample Papers

Entrance Exams Preparation

(a) Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
(b) Write the use of restriction endonuclease in the above process.