Question
How has RNAi technique helped to prevent the infestation of roots in tobacco plants by a nematode Meloidegyne incognita?
Solution
The introduction of DNA using the Agrobacterium vectors into the host plant produced both sense and anti-sense RNA in the host cells. The RNAi (RNA interference method was initiated as the two RNA's were complementary to each other and formed double-stranded RNA. The dsRNA silenced the specific mRNAof the nematode and thus the parasite could not survive in the transgenic host plant. The plant was therefore protected from the parasite.
