How did an American Company. Eli Lilly use the knowledge of r-DNA technology to produce human insulin?

Solution

American Company Eli Lilly used the knowledge of r-DNA technology to produce human insulin in the following way.

1. Preparation of DNA corresponding to A and B chain separately
2. Extraction of plasmid from bacteria
3. Insertion of DNA corresponding to A and B chain separately in plasmid
4. Transforming the bacteria with this recombinant plasmid
5. Expression of the desired product from this DNA .
6. Purification of A and B chain
7. Linking them with disulfide bonds so that they act as human insulin

Some More Questions From Biotechnology and Its Applications Chapter

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Biotechnology And Its Applications

How did an American Company. Eli Lilly use the knowledge of r-DNA technology to produce human insulin?

Some More Questions From Biotechnology and Its Applications Chapter

Bt-toxin protein exist in which form ?

How is inactive Bt-toxin converted into active form ?

How Bt toxin causes death of insect ?

Name a few forms of cry gene.

List the specific insects killed by (i) cryIAc and (ii) cryIAb.

Name the insect killed by proteins coded by cryIIAb and cry IAc.

What is unique about transgenic animals ?

What is silencing of mRNA ?

What is the process of silencing mRNA ?

Name the genetically engineered insulin.

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