Question
(a) State the role of DNA ligase in biotechnology.
(b) What happens when Meloidegyne incognitia consumes cells with RNAi gene?Solution
(a) DNA ligase is an enzyme which is used to join two DNA fragments.
(b) When Meloidogyne incognita (parasite) consumes cells with RNAi gene, the introduced RNAi gene forms both sense and anti-sense RNA and the two strands being complementary to each other form ds RNA, which cannot be transcribed. Thus, the mRNA of nematode is silenced and the parasite cannot survive there.
