Question
Can you suggest a method to remove oil (hydrocarbon) from seeds based
on your understanding of rDNA technology and chemistry of oil?
Solution
The genes for the formation of glycerol or fatty acids which are the main constituents of oil, should be identified and removed from the seed by using enzyme restriction endonucleases. Such DNA molecule should then be treated with DNA ligases to join the broken ends. These cells when grown in a minimum nutrient medium, under aseptic conditions will differentiate into a new variety whose seeds will not have oil in them because the gene producing the oil has been removed.