Question
How is ELISA test carried out ?
Solution
ELISA or Enzyme Linked Immunosorbent Assay is for detection in immunology . It is based on the principle of antibody antigen interactions.
Steps of ELISA
1. A micro titer plate is coated with antigen.
2. Primary antibody specific to antigen for diagnosis is added to the plate. The antibody interacts with the antigen. then the plate is washed to remove any extra free antibody.
3. Secondary antibody which has a tag is then added to the well. The secondary antibody attaches to the primary antibody. The plate is washed to remove free secondary antibody.
4.Chromagen is added which produces color and aids in detection.
