Question
How is isolated gene of interest amplified ?
Solution
Gene of Interest is amplified using Polymerase chain reaction. This technique involves three main steps :
(i) Denaturation in which the double-stranded DNA is denatured by using high temperatures
(ii) Primer annealing - in this step two sets of primers are used that are complementary to the segment of DNA of interest. the lower temperature favors the attachment or annealing of primer to the DNA strands.
(iii) Extension of primers in which DNA polymerase enzyme is used to make copies of DNA .
