Question
List the steps in the formation of rDNA.
Solution
Steps in formation of rDNA
Recombined DNA technology involves the following steps :
(i) Isolation of DNA.
(ii) Cutting of DNA by restriction endonucleases.
(iii) Isolation and amplification of the gene of interest.
(iv) Ligation of the DNA fragment into a vector using DNA ligase.
(v) Transfer of DNA fragment into host cell.
(v) Culturing of host cell and extraction of the recombinant product.
