Question
Write a note on cloning vector.
Solution
Cloning Vectors is small DNA molecule which carries foreign DNA into the host cell and replicates and produces copy of the desired gene in the host organism.
Good cloning vectors have certain features -
(i) Origin of replication (Ori) so that it can replicate autonomously.
(ii) Selectable marker- so that isolation of the transformed cell is easy.
(iii) Cloning (Recognition) site so that DNA can be inserted into it.
(iv) Small size of vector.
