Question
Define vector. Give the properties of a “Good Vector”.
Solution
A vector is a DNA molecule that has the ability to replicate in an appropriate host cell, and into which the DNA fragment to be cloned is integrated for cloning.
A good vector must have the following properties :
A good vector must have the following properties :
(a) It should be able to replicate autonomously. It should have an Origin of replication.
(b) It should have selectable markers so that it can be easily isolated .
(c) It should have recognition site preferably a single site for restriction enzymes so that introduction of DNA is easy.
