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Molecular Basis Of Inheritance

Question
CBSEENBI12000349

List the steps involved in DNA fingerprinting.

Solution
Steps of DNA Fingerprinting are :

(i) DNA is isolated.
(ii) DNA is digested by restriction endonucleases,
(iii) The fragments od DNA are seperated by electrophoresis,
(iv) The fragments are then traferred to to synthetic membranes, such as nitrocellulose or nylon.
(v)It is hybridized using labelled VNTR probe.
(vi) Hybridised DNA fragments are detected by autoradiography.