Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the breakdown of fungal cell, plant cell and bacterial cell respectively?

Lysozyme, lipases, trypsin

Chitinase, cellulase, lysozyme

Chitinase, cellulase, trypsin

Trypsin, lipases, cellulase

Solution

Chitinase, cellulase, lysozyme

Process of RDT or Recombinant DNA Technology involves isolation of DNA of a desired DNA fragment. The DNA should be in pure form, free form other macro- molecules.

The bacterial cell or plant or an animal tissue is treated with enzyme lysozyme or cellulase or chitinase (fungal) enzyme respectively in order to break the cell to release DNA. RNA can be removed by treated the cell with ribonuclease and proteins can be removed by treatment with protease.