Question
Which organism is used to transfer T-DNA
Streptomyces hygroscopicus
Agrobacterium tumefaciens
Salmonella typhi
Escherichia coli
Solution
B.
Agrobacterium tumefaciens
Ti plasmid (tumour inducing) from the soil bacterium Agrobacterium tumefaciens is effectively used as vector for gene transfer to plant cells. The part of Ti plasmid transferred into plant cell DNA, is called the T-DNA. This T-DNA with desired DNA spliced into it, is inserted into the chromosome of the host plant where it produces copies of itself, by migrating from one chromosomal position to another at random.
