Question
Which of the following statements is false regarding SDS-polyacrylamide gel electrophoresis?
Proteins are separated by molecular weight
SDS is a detergent which gives charge to protein
Large protein move more slowly through gel
SDS is used to maintain the 3-dimensional structure of protein
Solution
D.
SDS is used to maintain the 3-dimensional structure of protein
For protein, SDS (sodium DOdecyl Sulphate) is an anionic detergent applied to protein sample linearize proteins and to impart a negative charge to linearized proteins.
