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Reproduction In Organisms

Question
CBSEENBI12047391
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The component of bacteria that retains the crystal violet stain during Gram staining is

  • O- antigen

  • lipopolysaccharide

  • peptidoglycan

  • cytoplasmic membrane

Solution

C.

peptidoglycan

Gram staining is the method of differentiating bacterial species into two large groups, i.e., Gram positive and Gram negative bacteria based on the physical properties of their cell walls.

In the process of Gram staining 

- alcohol is used for decolourlisation process. It solubilises lipid but does not solubilise peptidoglycan layer.

As Gram positive bacteria has less lipid percentage as compared to peptidoglycan. Thus, peptidoglycan which does not get solubilised retain the Gram stain and Gram negative bacteria which has thinner peptidoglycan layer cannot retain the Gram stain.

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