Which of the following statements is/are correct regarding the effects of pH on enzyme catalysed reactions?
Direction of the reaction is influenced by [H+]
Ionisation state of dissociating groups on the enzyme is modified
Ionisation state of the substrate is modified.
Protein is not denatured with the change in pH
A.
Direction of the reaction is influenced by [H+]
B.
Ionisation state of dissociating groups on the enzyme is modified
C.
Ionisation state of the substrate is modified.
Most proteins and therefore enzymes are active only within a narrow pH range between 5 and 9. pH is defined as the -log[H+] i.e., the direction of the reaction is influnced by [H+]. It also effects the ionisation state ofsubstrate, amino acid residues, dissociating groups on the enzyme. Extremes of pH which is not optimal for enzyme catalysed reactions to take place leads to denaturation of proteins.
