The chemiosmotic coupling hypothesis of oxidative phosphorylation proposes that adenosine triphosphate (ATP) is formed because
a proton gradient forms across the inner mitochondrial membrane
there is a change in the permeability of the inner mitochondrial membrane towards adenosine diphosphate (ADP).
high energy bonds are formed in mitochondrial proteins
ADP is pumped out of the matrix into the intermembrane space
A.
a proton gradient forms across the inner mitochondrial membrane
The chemiosmotic hypothesis was given by Mitchell. It explains the process of ATP formation and states that it is linked to development of proton gradient across the mitochondrial membrane. ATP synthase is required for ATP synthesis is located in F1 particles. It becomes active only when there is high concentration of proton on F0 side as compared to F1 side.
