A student wishes to study the cell structure under a light microscope having 10X eyepiece and 45X objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution?
Yellow
Green
Blue
Red
C.
Blue
Resolving Power or resolution is the ability of the lens to distinguish fine details and structure. It is the ability of a lens to differentiate between two points at a specified distance apart.
Resolving Power =
It depends upon 2 factors:
(i) Wavelength of light used for illumination
(ii) Power of objective lenses
Among Yellow, green, red and blue light; Blue colour has the shortest wavelength so, it will give best resolution.