D.

It remains active at hight temperature

Polymerase Chain Reaction (PCR) is used to amplify a DNA segment or to synthesize in vitro the multiple copies of gene (or DNA) of interest, using two sets of primers and the enzyme DNA polymerase. This enzyme is isolated from a bacterium Thermus aquatics and it remains active during the high temperature but high temperature induced denaturation of double stranded DNA

C.

DNA sequence

The steroid hormone receptor protein complex activate transcription of target gene by binding to specific DNA sequence.

D.

probe

Probes are 15-30 bases long radioactive labelled oligonucleotides (RNA or DNA) used to detect complementary nucleotide sequences, used for diseases diagnosis etc. 

B.

m-RNA

In the process of protein synthesis, the messenger RNA (m-RNA) is responsible for carrying the genetic code transcribed from DNA to specialized sites within the cell (called ribosomes) where the information is translated into protein composition. The sequence of amino acids in a particular protein is determined by the sequence of t-RNA, c-DNA or  r-RNA do not decide the amino acid sequence in protein synthesis.

D.

PCR

Southern hybridization is a technique used in molecular biology for detection of a specific DNA sequence in DNA samples in which DNA sequence in DNA samples in which excepting PCR we use all three methods such electrophoresis, blotting and autoradiography. Because PCR is the method used for the amplification of DNA sample. In vitro, clonal propagation is characterised by PCR and RPAD.